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Monolithic capillary column with integrated electrochemical detector
Authors: Komendová Martina | Metelka Radovan | Urban Jiří
Year: 2016
Type of publication: článek ve sborníku
Name of source: 23th Young Investigators’ Seminar on Analytical Chemistry : book of proceedings
Publisher name: University of Novi Sad
Place: Novi Sad
Page from-to: 21-25
Titles:
Language Name Abstract Keywords
cze Monolitická kapilární kolona s integrovaným elektrochemickým detektorem Kombinace monolitických kapilárních kolon a elektrochemické detekce nabízí elegantní způsob přípravy miniaturizovaných, levných a přenosných analytických systémů. Je představena miniaturizace a integrace electrochemického detektoru s použitím uhlíkové mikroelektrody jako pracovní a stříbrného mikrodrátu jako referentní elektrody. Připravené zařízení bylo testováno při analýze dopaminu v moči. Integrovaná monolitická kapilární kolona je prvním krokem ve vývoji miniaturizovaných analytických systémů umožňujících chromatografickou separaci na polymerních monolitech a elektrochemickou detekci. Monolitická kolona; kapilární HPLC; mikroelektrody; neurotransmitery
eng Monolithic capillary column with integrated electrochemical detector Fast and simple quantification of neurotransmitters in biological samples plays crucial role in diagnostic of several neurological diseases, for example depression, Parkinson’s disease, and others. A combination of monolithic capillary columns and electrochemical detection offers an elegant way in the preparation of miniaturized, cheap and portable analytical systems. Here we show miniaturization and integration of an electrochemical detector. Microelectrodes were glued to copper wire and then integrated into monolithic capillary column. Carbon fibers with diameter 7 μm and different surface treatment (epoxy sized and unsized) were used as working microelectrodes. As a reference microelectrode, either carbon fiber with coated metallic silver or silver microwire (diameter 25 μm) was employed. The final arrangement of detector utilized silver microwire as a reference electrode, which possessed better potential, chemical and mechanical stability in time, and unsized carbon fiber as a working electrode. Prepared electrochemical detector was characterized by amperometric detection of dopamine solutions. Detection limit of 110 pg of injected dopamine was achieved. Monolithic capillary column with integrated electrochemical detector was used for analysis of standards of neurotransmitters. Currently, the capabilities of prepared device are tested in analysis of dopamine in urine. Integrated monolithic capillary column is one of the first steps in the development of miniaturized analytical systems providing chromatographic separation with use of polymer monoliths and electrochemical detection. Such systems can be employed for a personalized medicine allowing reduction of both time and financial costs. Monolithic column; capillary HPLC; microelectrodes; neurotransmitters

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