University of PardubiceFaculty of Chemical Technology

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Mass Spectrometric Analysis of Dysregulated Lipids in Tumor Cells and Body Fluids

Authors: Holčapek Michal | Cífková Eva | Lísa Miroslav | Jirásko Robert | Khalikova Maria | Ovčačíková Magdaléna

Year: 2016

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Mass Spectrometric Analysis of Dysregulated Lipids in Tumor Cells and Body Fluids	Tumor cells and normal cells have different proteomic, meta bolomic and lipidomic composition. Lipids are major building blocks of membrane s of cells and intracellular compartments and they also fulfil numerous crucial cell fu nctions. Their dysregulation is related with some serious human diseases including cancer. In our research we demonstrated that the lipidomic composition of tumor and normal cell lines shows statistically significant differences for several tumor types, differences are also found in tumor and normal tissues for breast and kidney cancer and confirmed on lipidomic cha nges of plasma samples of inbred mice. The last and the most difficult step is the sear ch for statistically relevant lipidomic differences for human body fluids suitable for early d iagnosis, such as plasma, erythrocytes or urine. This research requires high-throughput, robust and v alidated mass spectrometry based methods for the quantitation of large number of l ipids from multiple lipid categories and classes.