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Color change of phenol red by integrated smart phone camera as a tool for the determination of neurotoxic compounds
Authors: Kostelník Adam | Čegan Alexander | Pohanka Miroslav
Year: 2016
Type of publication: článek v odborném periodiku
Name of source: Sensors
Page from-to: "1212-1"-"1212-10"
Titles:
Language Name Abstract Keywords
cze Barevná změna fenolové červeně snímaná chytrým telefonem jako nástroj pro stanovení neurotoxických látek Použití mobilního telefonu jako detekčního systému je jednoduché a nevyžaduje speciálně školený personál, v kontrastu se standardním laboratorním vybavením. Tato práce se zabývá imobilizací acetylcholinesterasy (AChE) do želatinové membrány, a využití fenolové červeně jako ukazatele aktivity AChE. AChE štěpí acetylcholin na cholin a acetát, který mění pH roztoku, což vede k barevné změně indikátoru. V přítomnost inhibitoru je barevná změna úměrná jeho koncentraci. Měření bylo prováděno na 3D tištěném držáku, a pořízené digitální fotografie byly analyzovány za použití red-green-blue (RGB) bodů. Byly sestaveny kalibrace pro AChE inhibitory, takrin a galantamin, s limity detekce 1,1 nM respektive 1,28 µM. Metoda byly validována za použití standarní Ellmanovi metody a postup ověřen na vzorcích plasmy. Acetylcholinesterasa; fenolová červeň; chytrý telefon; kapková analýza; kolorimetrie, inhibitor; biosenzor
eng Color change of phenol red by integrated smart phone camera as a tool for the determination of neurotoxic compounds The use of a cell phone as a detection system is easy, simple and does not require trained personnel, which is in contrast to standard laboratory instruments. This paper deals with immobilization of acetylcholinesterase (AChE) in a gelatin matrix, and phenol red, as an indicator of AChE activity, is used in order to establish a method that is easily compatible with a camera device. AChE splits acetylcholine into choline and acetic acid, which changes the pH of a medium, resulting in a phenol red color change. The coloration changed in presence of an AChE inhibitor. Measurements were performed on 3D-printed, tube-shaped holder, and digital photography, with subsequent analysis of red-green-blue (RGB), served for assay purposes. Calibration of AChE inhibitors, tacrine and galantamine, was performed, with limit of detection equal to 1.1 nM and 1.28 µM, respectively. Interferences were also measured, resulting in a proof-of-method stability. The method was further successfully validated for the standard Ellman’s assay, and verified on murine plasma samples spiked with inhibitors. Acetylcholinesterase; phenol red; smart phone; drop assay; colorimetry; inhibitor; biosensor

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