Publication detail

Analysis of Eicosanoids in Biological Samples Using the Coupling of Separation Techniques and Mass Spectrometry

Authors: Chocholoušková Michaela | Cífková Eva | Holčapek Michal

Year: 2017

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Analysis of Eicosanoids in Biological Samples Using the Coupling of Separation Techniques and Mass Spectrometry	Oxylipins, including eicosanoids, play an important role in the ongoing inflammation in the human body. Eicosanoids are formed by the enzymatic or non-enzymatic oxidation of arachidonic acid or other polyunsaturated fatty acids. For the analysis of oxylipins, we used an ultrahigh-performance liquid chromatography (UHPLC) coupled to negative-ion electrospray ionization mass spectrometry using triple quadrupole analyzer. Analytes were separated in reversed-phase mode using Acquity UPLC BEH C18 (2.1x150 mm, 1.7 μm) column. All important parameters, such as turbo ion spray source temperature, nebulizer, and turbo gas flow have been optimized to achieve the highest sensitivity for subsequent selected reaction monitoring (SRM) transitions. MS/MS spectra of eicosanoids show multiple product ions. The collision energy was optimized for each precursor / product ion pairs used for SRM detection. This method will be validated and applied to samples of human plasma prepared by solid-phase extraction (SPE) using C18 column.	Eicosanoids; Oxylipins; UHPLC; MS/MS

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