University of PardubiceFaculty of Chemical Technology

University

Employees
|
Students
Česky

Publication detail

Lipidomic Analysis in the Cancer Biomarker Research

Authors: Holčapek Michal | Khalikova Maria | Lísa Miroslav | Jirásko Robert | Cífková Eva | Wolrab Denise | Hájek Roman | Chocholoušková Michaela | Vrána David | Melichar Bohuslav

Year: 2017

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Lipidomic Analysis in the Cancer Biomarker Research	Tumor cells have different lipidomic composition in comparison to normal cells. These changes could be also reflected in collectable body fluids, which may be used in the research of cancer biomarkers. Lipids are building blocks of cell membranes and intracellular compartments, and they also fulfil numerous crucial cell functions, therefore the study of their dysregulation in cancer is quite promising [1-3]. The series of MS based liquid-phase separation techniques (various modes of UHPLC and UHPSFC) has been developed and fully validated for the quantitation of a wide range of lipid species from multiple lipid categories. Several analytical methods are essential due to rather different physico-chemical properties of individual lipid classes and also their natural abundances starting from major components (e.g., phospholipids, sphingolipids and glycerolipids) up to ultratrace levels (e.g., oxylipins) but with important biological functions and possible relation to the disease onset and progress. The major requirement for the reliable and robust lipidomic quantitation is the use of non-endogenous internal standards (IS) for all lipid classes to be quantified and also the coelution and coionization of lipid class IS and analytes using the lipid class separation approaches based on normal-phase or HILIC separation mechanisms or shotgun approaches without chromatographic separation. In specific cases, the requirement for the coelution of lipid class IS and analytes cannot be implemented due to large number of isomers to be separated, which results in essential use of reversed-phase (RP) mode, such as in case of oxylipins. The following lipidomic methods will be discussed in the presentation: UHPSFC/MS of nonpolar and polar lipid classes [4], HILIC-UHPLC/MS of polar lipid classes, HILIC-UHPLC/MS of gangliosides, and RP-UHPLC/MS of oxylipins [5].	Lipidomic analysis; Cancer; Biomarker