Publication detail

Multidimensional Liquid Chromatography

Authors: Jandera Pavel

Year: 2017

Type of publication: kapitola v odborné knize

Name of source: Encyclopedia of Analytical Chemistry

Publisher name: John Wiley & Sons Ltd.

Place: Chichester

Page from-to: 1-31

Titles:

Language	Name	Abstract	Keywords
cze	Vícerozměrná kapalinová chromatografie	V práci jsou popsány základy vícerozměrné kapalinové chromatografie včetně off-line uspořádání, sériového stop-and-go zapojení, comprehensive a hear-cutting módu s důrazem na rozlišení, píkovou kapacitu, efektivitu, orthogonalitu a selektivitu. Dále jsou zde popsány metody optimalizace experimentálních podmínek a výběr chromatografických kolon a kombinace jednotlivých typů chromatografie.	Vícerozměrná kapalinová chromatografie; comprehensive LC; orthogonalita; píková kapacita; přenos frakcí mezi dimenzemi
eng	Multidimensional Liquid Chromatography	Fundamentals of multidimensional liquid chromatographic techniques are overviewed, including off-line, serial, stop-and-go, and heart-cutting and comprehensive separation modes, with attention to resolution, peak capacity, efficiency, orthogonality, and selectivity with special attention to the effects of sample structure on the retention and optimization of two-dimensional (2-D) separation systems, including the selection of columns, flow rate, fraction volumes, and mobile phase.Experimental set-up, modulation of the fraction collection and transfer from the first to the second dimension, compatibility of mobile phases in comprehensiveLC×LC, and 2-D asymmetry are addressed. The survey presents various coupled 2-D modes (RP ×RP, HILIC ×RP, IEX×RP, and SEC × RP) and problems with their on-line coupling.	multidimensional LC; comprehensive LC; orthogonality; peak capacity; sample transfer modulation

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