Publication detail

The use of different substances in boar semen extender as a substitute for antibiotics and their effect on inhibition of microorganisms and sperm survival

Year: 2017

Type of publication: ostatní - článek ve sborníku

Name of source: Research in pig breeding : workshop proceedings of abstracts

Publisher name: Výzkumný ústav živočišné výroby, v.v.i.

Place: Praha

Page from-to: 10-11

Titles:

Language	Name	Abstract	Keywords
eng	The use of different substances in boar semen extender as a substitute for antibiotics and their effect on inhibition of microorganisms and sperm survival	Microorganisms contaminating boar semen are one of the most important factors that negatively affect the biological quality of boar semen. The objective this study was to investigate the effect different substances added to boar extender as a substitute for antibiotics and their influence on sperm survival. The concentrations of test substances used to extender BTS without antibiotic (BTS0) were following: zinc sulphate and sodium thiosulfate 0.75 g/l, 1 g/l and 1.25 g/l, colloidal zinc with ascorbic acid 8.54+100.69 mg/l and 17.08+201.38 mg/l, gallic acid 1.5 g/l (G1), 1.75 g/l (G2) and 2 g/l (G3) and honey 16 g/l (H). Then the boar semen was diluted (dilution rate of 1+2, 1+4 and 1+8) in extender BTS with antibiotic (BTSA) and BTS0 with different substances. Diluted samples were stored at 17°C up to 48h. In microbiological laboratory, total bacterial counts were determined using plate count method. The samples were processed for 3 subsequent days. Concentrations of test substances have not effect on mean initial sperm motility 83 % (p<0.05). Samples were taken for determination antibacterial activity of substances and sperm motility every day. Survival sperm was affected by storage time, the dilution ratio 1 + 8 and type of substances added to the extender (p<0.05). A statistical significant difference in total sperm motility were found between samples BTS 70.21 % vs. BTSO with sodium thiosulfate (1.5 g/l) 61.39 %, G2 57.87 % and G3 58.89 % vs. BTS 70.21 %, BTS0 70.37 % (p<0.05). The sample with the honey substance had statistically lower sperm motility 49.09 % compared to other test samples (p<0.05). The most frequently isolated microorganisms from boar semen were: E. coli, Proteus sp., Staphylococcus aureus, Bacillus sp. and Moraxella canis. Microorganisms weren´t significantly inhibited by any of tested substances added to the extender, namely colloidal zinc, sodium thiosulfate, gallic acid and honey.	boar semen; boar extender; antibacterial activity

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