Publication detail

Isotachophoretic determination of amino acids after their conversion to hydroxy acids

Year: 2018

Type of publication: článek v odborném periodiku

Name of source: Monatshefte fur Chemie

Publisher name: Springer

Place: Heidelberg

Page from-to: 1709-1718

Titles:

Language	Name	Abstract	Keywords
cze	Izotachoforetické stanovení aminokyselin po jejich konverzi na hydroxykyseliny	V tomto článku je prezentováno stanovení aminokyselin kapilární izotachoforézou na základě derivatizace (van Slykeova reakce). Proto navrhovaná metoda využívá reakci aminokyselin s kyselinou dusitou, při které vznikají tvoří odpovídající hydroxykyseliny. Vedoucí elektrolyt 0,01 M HCl s beta-alaninem (pH 3,6) a 0,05% hydroxyethylcelulózou. Koncový elektrolyt 0,01 M kyselina valerová s NaOH (pH 7,24).	aminokyseliny; izotachoforéza; stanovení
eng	Isotachophoretic determination of amino acids after their conversion to hydroxy acids	In this article, determination of amino acids by capillary isotachophoresis based on their derivatization (van Slyke reaction) is presented. Hence, the suggested method deals with reaction of amino acids with nitrous acid forming corresponding hydroxy acids. This reaction was used in isotachophoretic analysis for the first time. Optimal conditions represented by electrolyte system for separation of hydroxy acids, 0.01 mol/dm(3) HCl, 0.05% hydroxyethyl cellulose, with beta-alanine (leading electrolyte of pH 3.6) and 0.01 mol/dm(3) valeric acid with sodium hydroxide (terminating electrolyte of pH 7.24), were chosen; furthermore, other critical details of the analysis are also described. Developed method brings original results and shows applicability on analysis of amino acids in relatively simple mixtures, especially in food supplements. [GRAPHICS] .	Aminoacid; Isotachophoresis; Determination

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