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Lactofen - Electrochemical Sensing and Interaction with dsDNA

Authors: Guziejewski Dariusz | Morawska Kamila | Poplawski Tomasz | Metelka Radovan | Ciesielski Witold | Smarzewska Sylwia

Year: 2018

Type of publication: článek v odborném periodiku

Name of source: Electroanalysis

Page from-to: 94-100

Titles:

Language	Name	Abstract	Keywords
cze	Lactofen - elektrochemická detekce a interakce s DNA	Byla zkoumána elektrochemická redukce lactofenu na elektrodách ze skelného uhlíku a stříbrné filmové amalgámy s využitím square wave, rozpouštěcí square wave a cyklické voltametrie. Stříbrná amalgámová filmová elektroda vykazovala výraznou elektrochemickou aktivitu při redukci lactofenu a zvýšení citlivosti jeho stanovení oproti elektrodě ze skelného uhlíku. Dále byly studovány interakce lactofenu s dvojřetězcovou DNA izolovanou ze spermií lososa.	Lactofen; Square wave voltametrie; Elektroda ze skelného uhlíku; Stříbrná amalgámová filmová elektroda; Stanovení herbicidu; DNA; Interkalace
eng	Lactofen - Electrochemical Sensing and Interaction with dsDNA	The electrochemical reduction of lactofen (LCT) at the glassy carbon (GCE) and silver amalgam film electrode (AMFE) is investigatedin detail by the means of square wave voltammetry (SWV), square wave stripping voltammetry (SWSV) and cyclic voltammetry. The influence of various factors such as supporting electrolyte composition and SW parameters were studied. The AMFE electrode showed an excellent electrochemical activity toward the electro-reduction of LCT, leading to a significant improvement in sensitivity as compared to the glassy carbon electrode.The SWSV detection limits for GCE and AMFE were 285.0nM and 2.0nM, respectively. The applicability of the developed voltammetric method for analysis of tap water and river water is illustrated with spiked samples analysis. Moreover, as lactofen is highly toxic to fish and other aquatic organisms, its interaction with dsDNA isolated from salmon sperm was tested. The intercalative mode of LCT binding to dsDNA was estimated. The heterogeneous rate constants were calculated for the free LCT and the LCT-dsDNA complex. Moreover, LCT-dsDNA complex binding ratio and equilibrium constant were determined. The decrease in the SWV peak current of LCT in the presence of dsDNA was used for the determination of dsDNA.	Lactofen; Square-wave voltammetry; Glassy carbon electrode; Silver amalgam film electrode; Herbicide determination; DNA; Intercalation