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The annelid Enchytraeus crypticus cultivated in agar-based media as a convenient biological model for testing toxicity of engineered nanoparticles
Authors: Hrdá Kateřina | Pouzar Miloslav | Konopáčová Eliška
Year: 2019
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng The annelid Enchytraeus crypticus cultivated in agar-based media as a convenient biological model for testing toxicity of engineered nanoparticles The agar based cultivation media are able to suppress dynamic processes (as a particle agglomeration/de-agglomeration, sedimentation, dissolution/precipitation) usually occurring during the ecotoxicity tests of nanometrials on aquatic or terrestrial organisms. When agar gel is used, quasi-static conditions during the experiment together with the opportunity to analyze physicochemical status of particles very close to the contact point with the biological model offers data reflecting true nature of particle-organism interaction. By such way obtained results are able to clarify some discrepancies regarding the influence of particle physicochemical characteristics (size, agglomeration state, adsorbed impurities etc.) on ecotoxicity test results. Non supplemented agar provides the conditions, which are close to the aquatic or the pore water environments. Enrichment of agar with model soil constituents like kaolin or humic acids is the way, how to study particular nanoparticle-soil component interactions and their influence on the resulting dose-response relationships. Enchytraeus crypticus as a representative of soil invertebrates belongs to widely used test organisms. Its utilization gives us an opportunity to work with more complex (than single-cell) organism, which is easy to cultivate and analyze. We believe that rigorous approach based on suppression of dynamic changes of nanoparticles during the test and optimized procedure of exposure media analysis is able to provide valuable data not only for a mechanistic understanding of ENPs interaction with the environment and living organisms but also as the first step within the more complex tiered strategy of the environmental risk assessment of engineered nanoparticles. Agar; Enchytraeus crypticus; nanparticles; ecotoxicity; agglomeration

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