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Development of HPLC/MS/MS Method for Analysis of Biologically Active Compounds in Tropaeolum Majus
Authors: Česlová Lenka | Šalomounová Tereza
Year: 2019
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Development of HPLC/MS/MS Method for Analysis of Biologically Active Compounds in Tropaeolum Majus Tropaeolum majus is widely cultivated as an ornamental and medicinal plant. Its leaves, flowers and green seeds are edible with similar taste as horseradish. Due to the high content of biologically active compounds, the plant and its extracts are frequently used in traditional medicine to treat diseases including cardiovascular disorders, urinary tract infections, asthma and many others. The therapeutic effects of the plant are believed to be related to the high levels of glucotropaeolin, carotenoids, vitamin C and various phenolic compounds, especially flavonoids. This work is focused on analysis of phenolic compounds and glucotropaeolin in methanolic extracts of the plant using reversed-phase HPLC/MS/MS method. First, the HPLC separation and MS/MS detection was optimized to analyze selected phenolic compounds and glucotropaeolin in one analysis without its desulphation. The separation was performed on octadecyl silica gel column modified for separation of polar compounds packed with fused core particles. Very short separation of almost 40 compounds were achieved in 7 minutes using gradient elution with mobile phase consisted of acetonitrile and water acidified by formic acid to pH=2.5. The mass spectrometric detection in MRM mode was optimized for detection and quantitation of all identified biologically active compounds. The amount of the monitored compounds was determined in the extracts prepared from different parts of plant using 70% methanol. The influence of pretreatment of the sample after harvesting (freezing, drying) on the content of the monitored compounds was also monitored. tropaeolum majus, HPLC/MS/MS, extraction

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