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Vanadocene complexes bearing N,N'-chelating ligands: Synthesis, structures and in vitro cytotoxic studies on the A549 lung adenocarcinoma cell line
Authors: Melounková Lucie | Machálková Aneta | Havelek Radim | Honzíček Jan | Rezacova Martina | Císařova Ivana | Peterova Eva | Vinklárek Jaromír
Year: 2019
Type of publication: článek v odborném periodiku
Name of source: Journal of Inorganic Biochemistry
Publisher name: Elsevier Science BV
Place: Amsterdam
Page from-to: 182-193
Titles:
Language Name Abstract Keywords
cze Vanadocenové komplexy s N,N-chelátujícími ligandy: Příprava, struktura a in vitro aktivita na buněčné linii A549 Nové vanadocenové komplexy s N,N-chelatujícími ligandy byly připraveny a charakterizovány. byla měřena jejich cytotoxicita vůči panelu nádorových buněk. Struktura 4 sloučenin byla stanovena RTG analýzou. Mechanismus cytotoxického účinku byl studován na dvou derivátech. vanad; cytotoxicita; apoptóza
eng Vanadocene complexes bearing N,N'-chelating ligands: Synthesis, structures and in vitro cytotoxic studies on the A549 lung adenocarcinoma cell line Ten new vanadocene complexes bearing N,N'-chelating ligands were prepared, characterized, and their cytotoxicity toward a panel of cancer cells was measured. Structures of four vanadocene compounds were determined by single crystal X-ray diffraction analysis. Complexes containing 1,2-bis(phenylimino)acenaphthene (bian) and 1,2-bis(4-methoxyphenylimino)acenaphthene (4-MeO-bian) exhibit higher cytotoxicity than those with dipyrido[3,2-a:2',3'-c]phenazine (dppz) and (E)-N-((pyridin-2-yl)methylene)benzenamine (pyma). In light of the finding, cytotoxic mechanisms of two highly effective complexes [(eta(5)-C5H4Me)(2)V(bian)][OTf](2) (3b) and [(eta(5)-C5H4Me)(2)V(4-MeO-bian)][OTf](2) (4b) against human A549 lung adenocarcinoma cells were investigated by following membrane leakage of intracellular lactate dehydrogenase, Trypan Blue staining and activation of tumor protein p53 (p53). Evaluated complexes have a potent dose-dependent antiproliferative activity, causing cell cycle redistribution by the increased accumulation of cells in the G2 and S phase. In accord with the observed cell cycle deceleration, cyclin-dependent kinase inhibitor-interacting protein 1 (p21(WAF1/Cip1)), extra cellular signal regulated kinases 1 and 2 (ERK1/2), Checkpoint kinase 1 (Chk1), Checkpoint kinase 2 (Chk2) and their phosphorylated forms Chk1 at serine 345 and Chk2 at threonine 68 increased. In the cells exposed to complexes, dose- and time-dependent apoptotic process is initiated by the activation of the initiator caspase 8, followed by activation of effector caspase 3/7 and phosphatidylserine externalization. Moreover, because of treatment, A549 cells activate prosurvival mitogen-activated protein kinases (MAPK) signaling and up-regulate antiapoptotic protein B-cell lymphoma (Bcl-2), thereby promoting evasion of cell death. Both complexes exhibited considerably higher cytotoxic effect than the reference anticancer drug cis-platin and the cytotoxicity was more pronounced at higher treatment t Vanadium(IV); Cytotoxicity; Apoptosis; A549 lung adenocarcinoma cells

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