Publication detail

Comparison of Electrodriven Focusing Methods for Improvement of Two-dimensional Separations

Authors: Váňová Jana | Sadílková Jaroslava | Česla Petr

Year: 2019

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Comparison of Electrodriven Focusing Methods for Improvement of Two-dimensional Separations	Two-dimensional separation system consisting of liquid chromatography in the first dimension and capillary electrophoresis in the second dimension gives a lot of benefits. In the field of separation techniques, capillary electrophoresis brings the advantages such as high efficiency, short analysis time, low reagent and sample consumption. On the other hand, the sensitivity of the most commonly used UV/Vis detection is low due to small sample injection volume and short optical path length. The sample from the first dimension is usually diluted and improving the sensitivity of the detection is thus necessary. There are several on-line options to improve the sensitivity of the capillary electrophoresis with UV/Vis detection. The method can be based on the migration changes of the analyte between the sample zone and background electrolyte, on the sweeping by using micelles of the surfactants, or on the ionic strength-mediated stacking. This work presents the comparison of the selected methods intended for application in two-dimensional LC-CZE method. The model sample of phenolic acids was prepared and the preconcentration techniques were tested, the enhancement factors were calculated and the results were compared.

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