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UHPLC Analysis and Antioxidant Activity of Carob Powder
Authors: Frühbauerová Michaela | Červenka Libor | Hájek Tomáš | Pouzar Miloslav | Palarčík Jiří
Year: 2019
Type of publication: ostatní - přednáška nebo poster
Name of source: YISAC 2019 : book of abstracts
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 20
Titles:
Language Name Abstract Keywords
eng UHPLC Analysis and Antioxidant Activity of Carob Powder Work was focused on optimization of extraction of polyphenolic compounds from carob powder after grinding on different grinders, cryogenic and vibratory (4 and 8 minutes of grinding on this grinder type). Each powder was extracted to methanol (90:10 with water (v/v), acidified by 15 µl of formic acid). In the next step, the method for analysis of compounds in carob powder using liquid chromatography with reversed-phase (Kinetex XB-C18 100Å column, 150×2.1 mm, particle size 1.7 µm) was optimized. The mobile phase was formed by mixture of acetonitrile and deionized water with addition of formic acid (pH ~ 3.1). Gradient elution for separation was used. Diode array detector (DAD) was employed for detection (λ = 270, 290 and 320 nm). Eight compounds (vanillic acid, ferulic acid, cinnamic acid, quercitrin, luteolin, naringenin, apigenin and chrysoeriol) were found in all samples. The highest amount of these compounds was detected in extract from powder after cryogenic grinding. Determination of antioxidant activity of carob extracts by DPPH and ABTS method was part of this work too. Results were reported as Trolox equivalent (TE). For extract from carob powder after cryogenic grinding, 15.60 ± 0.87 mg TE/g of powder (DPPH method) and 28.58 ± 2.92 mg TE/g of powder (ABTS method) were found. Regarding the extract of carob powder (vibratory grinding for 4 min), 9.06 ± 0.60 mg TE/g of powder (DPPH method) and 17.31 ± 1.28 mg TE/g of powder (ABTS method) were determined. Preparation of carob powder by vibratory grinding for a longer period of time (i.e. 8 min) resulted in the increase of antioxidant activity in terms of DPPH (13.21 ± 0.63 mg TE/g of powder) and ABTS methods (22.82 ± 1.77 mg TE/g of powder) in comparison with vibratory grinding for 4 min. All results are shown as arithmetic mean with confidence interval (level of significance 0.05). carob; DPPH; grinding

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