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Optimization steps of mucosal vaccine preparation using polymeric nanoparticles
Authors: Slováková Marcela | Behančínová Petra | Bolková Alexandra | Janovská Sylva | Sleha Radek
Year: 2022
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Optimization steps of mucosal vaccine preparation using polymeric nanoparticles In recent years, there has been growing interest in the use of nanomaterials as drug carriers, mainly because of the benefits attributed to these materials. Polymer nanoparticle systems are capable of local and sustained administration for drug delivery, facilitate the improvement of the therapeutic index of drugs, and are capable of controlling drug release1. Proteins containing chitosan nanoparticles have potential in the field of targeted drug transport to the mucosa2. One of the parameters studied in the development of immobilized drug nanoparticles is the in vitro evaluation of drug release from a polymeric carrier, which is typically achieved by controlling the rate of polymer biodegradation (erosion) and drug diffusion from the polymer matrix3. Therefore, we aimed to find a suitable system with a high protein load and slow protein release. Here we show the preparation of nanoparticles from chitosan-TPP formed by ionotropic gelation and modified with PCL or hyaluronic acid and proteins (ovalbumin, epidermal growth factor). We characterized the prepared chitosan-based nanoparticles by measuring the DLS and the zeta potential. We then tested different methods of protein binding (encapsulation, adsorption, or chemical binding with carbodiimide) to chitosan-based nanoparticles. The mean nanoparticle size for chitosan and chitosan-PCL was 221.0 ± 46.61 nm (PI 0.52) and 231.9 ± 20.3 (PI 0.64) with a positive zeta potential of 41.69 mV and 36.72 mV. The binding efficiency using the BCA protein quantification assay was 45.39%, 44.2%, and 23.9% for chitosan-TPP protein encapsulation, chitosan-TPP protein sorption, and chitosan-PCL, respectively. Less than 10% of the protein was released from the particles in different pH buffers in the first 15 minutes, and then the release rapidly slowed. The results showed that chitosan-based nanoparticles with proteins could be suitable as a drug delivery system. mucosal; vaccine; chitosan; nanoparticles

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