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Determination of α-tocopherol in different biological samples by GC-MS without derivatization
Authors: Kopčil Michal | Janeček Radim | Kanďár Roman
Year: 2022
Type of publication: článek v odborném periodiku
Name of source: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
Page from-to: 51-59
Titles:
Language Name Abstract Keywords
cze Stanovení α-tokoferolu v různých biologických vzorcích metodou GC-MS bez derivatizace Byla vyvinuta metoda pro stanovení hladiny α-tokoferolu v lidské plazmě a tkáni tlustého střeva metodou plynové chromatografie s hmotnostní detekcí. Vzorky byly získány od 27 pacientů s Crohnovou nemocí. Vzorky byly deproteinovány, α-tokoferol extrahován ve dvou krocích a jeho hladina stanovena metodou GC-MS bez předchozí derivatizace. Analytické parametry metody byly vyhovující: variační koeficienty přesnosti v sérii a mezi sériemi byly pod 7 % a výtěžnost se pohybovala v rozmezí 92,2-100,5 %. Linearita metody byla dosažena pro rozmezí 0,6-64,3 μmol/l. Medián hladiny α-tokoferolu v plazmě byl 21,6 μmol/l, zdravé tkáni tlustého střeva 26,5 nmol/mg a tkáně ovlivněné Crohnovou nemocí 34,9 nmol/mg. Nenalezli jsme statisticky významnou korelaci mezi hladinami α-tokoferolu v plazmě a tkáni. Naše metoda má potenciál být zahrnuta do studií monitorující α-tokoferol jako ukazatel oxidačního stresu. α-tokoferol; Crohnova nemoc; tkáň tlustého střeva; GC-MS
eng Determination of α-tocopherol in different biological samples by GC-MS without derivatization A method has been developed to determine the levels of α-tocopherol in human plasma and colon tissue using gas chromatography with mass spectrometric detection. The samples were acquired from 27 patients with Crohn’s disease. Samples were deproteinized and α-tocopherol was obtained using a two-step extraction and measured by GC-MS without prior derivatization. The analytical parameters of the method were satisfactory: the intra-day and inter-day coefficients of variation were below 7% and quantitative recoveries range between 92.2-100.5%. The method shows an excellent linear range from 0.6-64.3 μmol/L. The median measured α-tocopherol concentration in human plasma was 21.6 μmol/L and in healthy colon tissue and tissue affected by Crohn’s disease was 26.5 nmol/mg and 34.9 nmol/mg, respectively. We did not find any statistically significant correlation or differences between the α-tocopherol content in plasma and tissue. Overall, our method has potential to be easily implemented in special studies for monitoring α-tocopherol as a biomarker of oxidative stress. α-tocopherol; Crohn´s disease; colon tissue; GC-MS

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