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In vitro activity of avocado oil extracts against planktonic cells and biofilm formation of Arcobacter-like species
Year: 2022
Type of publication: ostatní - článek ve sborníku
Name of source: Hot Topics in Microbiology - International Conference for Young Scientists : sborník příspěvků
Publisher name: Univerzita Komenského v Bratislave
Place: Bratislava
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng In vitro activity of avocado oil extracts against planktonic cells and biofilm formation of Arcobacter-like species Arcobacter-like species are Gram-negative curved rods. Various species are considered emerging food pathogens and can cause human gastrointestinal disease. Arcobacter-like bacteria have been isolated from a variety of sources, including wastewater, meat, and seafood. Detection and identification of Arcobacter-like species by biochemical methods are problematic, and therefore, molecular biological methods are used. These bacteria are able to form biofilm structures. The biofilm formation ability results in greater resistance to antimicrobial substances. Vegetable oils have been tested for antimicrobial effects. The aim of the study was to test in vitro effect of an avocado oil water extract on cell survival and biofilm formation. Minimum inhibitory concentrations (MIC) were determined and growth curves were measured using the RTS-1C bioreactor. The biofilm formation ability at different concentrations of extract was assessed using the modified Christensen method. Significant inhibition of Arcobacter-like species was especially observed up to a extract concentration of 90%. The exception was A. thereius LMG 24488, for which the MIC value of 45 % was determined. The highest biofilm formation was measured at concentrations of 0.00–0.35 %. A. defluvii LMG 25694 produced the most biofilm in all tested concentrations of avocado oil extract. On the contrary, the lowest amount of biofilm was observed for A. cryaerophilus CCM 7050. The results indicate that avocado oil extract has an antimicrobial effect that can affect the growth and survival of Arcobacter-like species. Antimicrobial effect; Arcobacter-like; avocado oil extract; biofilm formation

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