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The Effect of Antibiotics on Planktonic Cells and Biofilm Formation Ability of Collected Arcobacter-like Strains and Strains Isolated within the Czech Republic
Authors: Švarcová Karolína | Pejchalová Marcela | Šilha David
Year: 2022
Type of publication: článek v odborném periodiku
Name of source: Antibiotics
Publisher name: MDPI AG (Multidisciplinary Digital Publishing Institute)
Place: Basel
Page from-to: 87
Titles:
Language Name Abstract Keywords
cze Vliv antibiotik na planktonní buňky a schopnost tvorby biofilmu Arcobacter-like kmenů izolovaných v České republice Účelem této studie bylo otestovat in vitro účinky ampicilinu, ciprofloxacinu, klindamycinu, erythromycinu, gentamicinu a tetracyklinu na planktonní buňky Arcobacter-like species a na jejich schopnost tvorby biofilmu. Minimální inhibiční koncentrace (MIC) byly stanoveny mikrodiluční metodou. Schopnost tvorby biofilmu v přítomnosti různých koncentrací antibiotik byl hodnocen modifikovanou Christensenovu metodou. Většina z 60 kmenů vykazovala vysokou citlivost vůči gentamicinu (98,3 %), ciprofloxacinu (95,0 %) a erythromycinu (100,0 %). Vysoká úroveň rezistence byla pozorována ke klindamycinu a tetracyklinu s MIC50 a MIC90 v rozmezí 4–32 mg/l, resp. 32–128 mg/l. Kombinovaná odolnost vůči klindamycinu i tetracyklinu byly nalezeny u 38,3 % testovaných kmenů. Obecně vyšší tvorba biofilm byla pozorována zejména při nižších koncentracích antibiotik (0,13–2 mg/l). Byl však prokázán také významný pokles schopnosti tvořit biofilm Pseudarcobacter defluvii LMG 25694 v priostředí ampicilinu a klindamycinu v koncentracích nad 32, resp. 8 mg/l. Tvorba biofilmu představuje potenciální nebezpečí, a to zejména z důvodu rezistentních kmenů a schopnost tvořit biofilmy v prostředí nižších koncentrací antibiotik. antibiotika; resistance; MIC; biofilm; Arcobacter-like; Aliarcobacter butzleri; Aliarcobacter cryaerophilus
eng The Effect of Antibiotics on Planktonic Cells and Biofilm Formation Ability of Collected Arcobacter-like Strains and Strains Isolated within the Czech Republic The purpose of this study was to test the in vitro effects of ampicillin, ciprofloxacin, clindamycin, erythromycin, gentamicin, and tetracycline on planktonic cells of Arcobacter-like microorganisms and on their biofilm formation ability. The minimum inhibitory concentrations (MICs) were determined by the microdilution method. Further, biofilm formation ability in the presence of various concentrations of antibiotics was evaluated by a modified Christensen method. Most of the 60 strains exhibited high susceptibility to gentamicin (98.3%), ciprofloxacin (95.0%), and erythromycin (100.0%). High level of resistance was observed to clindamycin and tetracycline with MIC50 and MIC90 in range of 4-32 mg/L and 32-128 mg/L, respectively. Combined resistance to both clindamycin and tetracycline was found in 38.3% of tested strains. In general, higher biofilm formation was observed especially at lower concentrations of antibiotics (0.13-2 mg/L). However, a significant decrease in biofilm formation ability of Pseudarcobacter defluvii LMG 25694 was exhibited with ampicillin and clindamycin at concentrations above 32 or 8 mg/L, respectively. Biofilm formation represents a potential danger of infection and also a risk to human health, in particular due to antimicrobial-resistant strains and the ability to form a biofilm structure at a concentration that is approximately the MIC determined for planktonic cells. antibiotics; antibiotic resistance; MIC; biofilm; Arcobacter-like; Aliarcobacter butzleri; Aliarcobacter cryaerophilus

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