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GLYCOSPHINGOLIPIDOMICS Mass Spectrometry Profiling of Glycosphingolipids in Biological Samples

Authors: Hořejší Karel | Lásko Zuzana | Kolářová Denisa | Chocholoušková Michaela | Wolrab Denise | Jirásko Robert | Holčapek Michal

Year: 2024

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	GLYCOSPHINGOLIPIDOMICS Mass Spectrometry Profiling of Glycosphingolipids in Biological Samples	Glycosphingolipids (GSLs) are a diverse and structurally complex class of lipids that play crucial roles in various biological processes, including cellular signaling, recognition, and adhesion. They are integral components of cellular membranes and are implicated in the pathophysiology of numerous diseases, including cancer. The structural diversity of GSLs given by both the hydrophobic and hydrophilic part of the molecule poses significant challenges for their comprehensive analysis. High performance liquid chromatography coupled to mass spectrometry techniques have proven to be the best suited for the detection, structural elucidation, and quantitation of GSLs. Specifically, hydrophilic interaction liquid chromatography (HILIC) coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS) have enabled detailed structural profiling of GSL species from complex biological matrices such as human plasma/serum or tissues together with the separation of glycan isomers using porous graphitic carbon columns. Despite these advancements, efficient isolation and detection of especially low-abundance GSLs species still remain demanding. Addressing these issues is crucial for high-throughput GSL analysis, which holds promise for advancing our understanding of GSL roles in health and disease and for the development of novel diagnostic and therapeutic approaches.