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Using Multiparametric Surface Plasmon Resonance to determine the affinity of interaction between antigen and antibody

Authors: Novák Dominik | Morávek Ondřej | Kastrati Gylxhane | Matějková Nikola | Korecká Lucie

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Using Multiparametric Surface Plasmon Resonance to determine the affinity of interaction between antigen and antibody	Information about the affinity is crucial in various applications based on specific interaction between biomolecules, such as antigen and antibody, antigen and aptamer, enzyme and inhibitor etc. These applications include affinity biosensors, enzyme-linked immunosorbent assay (ELISA), drug design and screening, and many others [1]. These applications have gained relevant attention due to their expanding applications in biochemical detection, clinical diagnostics, food safety assessment and etc. The affinity and appropriate reactivity of biomolecules are essential for ensuring assay selectivity and sensitivity [1-2]. Several methods are used for studying this interaction, such as Western-blot, Bio-layer interferometry, ELISA, or Surface plasmon resonance (SPR) [2]. SPR is a label-free, optical biosensing technique that enables real-time monitoring of biomolecular interactions. SPR detects changes in the refractive index at the interface between a dielectric medium and a thin metal film, typically gold, as a result of analyte binding to the sensor surface. The major advantages of SPR include its label-free nature, minimal sample preparation, and potential for miniaturization and efficient screening [3–4]. Multiparametric SPR (MP SPR) is an enhanced version of SPR. Its improved goniometric design allows scanning over a wider range of refractive indices than standard SPR, enabling measurement of thicker layers and more complex systems [5]. This work was focused on the use of MP-SPR method for measuring of the interaction of specific antibodies with protein biomarker Pentraxin 3 associated with the intraamniotic inflammation. We also concentrated on the interaction of specific antibodies with protein HE-4 associated with ovarian carcinoma. We compared different types of modifications of gold sensors for the binding of antibodies.	Multiparametric Surface Plasmon Resonance (MP-SPR); Gold sensors; Antibody; Antigen; Affinity interaction