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Effect of Mobile Phase Composition on Retention Behavior of Metabolites in Bioinert LC Analysis

Authors: Langová Alena | Li Liang | Holčapek Michal

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Effect of Mobile Phase Composition on Retention Behavior of Metabolites in Bioinert LC Analysis	Hydrophilic interaction liquid chromatography coupled to mass spectrometry is a powerful and versatile technique widely used in metabolomics for the comprehensive study of small, polar, and ionizable molecules in biological systems. Polar metabolites may contain a chromatographically difficult functional group resulting in poor peak shape and low sensitivity. Bioinert systems with the optimized mobile phase composition can improve the chromatography of metabolites containing phosphate groups.	Mobile Phase; Composition; Retention behavior; Metabolites; Bioinert analysis