University of PardubiceFaculty of Chemical Technology

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Can we combine high-throughput and comprehensive lipidomic quantitation?

Authors: Holčapek Michal | Peterka Ondřej | Peroutková Petra | Jirásko Robert | Lásko Zuzana | Šubrtová Veronika

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Can we combine high-throughput and comprehensive lipidomic quantitation?	The growing importance of lipidomics in biomedical and clinical research increases the demand for analytical approaches that are both high-throughput and capable of delivering detailed, quantitative molecular information. This lecture addresses whether it is feasible to integrate rapid, large-scale analytical workflows with comprehensive lipid quantitation across diverse lipid classes. Key aspects include optimization of sample preparation, the use of advanced separation techniques such as UHPLC and SFC/MS, and strategies to shorten analysis time without compromising structural specificity or quantitative accuracy. The presented results demonstrate that the combination of high-throughput performance and in-depth lipidomic coverage is achievable through modern mass spectrometry platforms and standardized protocols, enabling robust quantification of a wide range of lipid species in extensive clinical cohorts	lipidomics; high-throughput analysis; lipid profiling; UHPLC; SFC/MS; mass spectrometry