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Characterisation of EGF Containing Chitosan Submicroparticles and Study of Internalisation in the A549 Cell Line

Authors: Slováková Marcela | Královec Karel | Komárková Kateřina | Štefanová Kateřina | Janovská Sylva | Sleha Radek

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Characterisation of EGF Containing Chitosan Submicroparticles and Study of Internalisation in the A549 Cell Line	Current pharmaceutical research is increasingly focused on the development of systems that enable the targeted and controlled delivery of bioactive substances, including proteins, in the field of wound healing and oncology therapy. Proteins have significant therapeutic potential due to their high specificity and biological efficiency, but their use might be limited by low stability and short biological half-life. To overcome these obstacles, a carrier based on chitosan (CS) was developed, a biocompatible, antimicrobial and non-immunogenic carrier with potential for antitumor and regenerative effects.1 CS particles with EGF were studied to ensure protein protection, stability, and effective release. The particles showed suitable physicochemical properties, DLS size up to 180 and 200 nm with protein, low PI, high positive zeta potential and flawless short-term stability of the properties. The particles efficiently captured the protein in the polymer matrix (86-87%) with a carrier binding capacity of approximately 65 μg/2 mg. The hyaluronic acid coating of the particles increased DLS, changed the zeta potential to negative values, and ensured a comparably efficient capture of EGF in the particles (89%). The results of protein release from lyophilised particles showed a biphasic release typical of CS particles, with a much lower protein release rate. The internalisation of FITC-labelled CS particles with tumour A549 cells, overexpressing the EGFR receptor, was tested with the aim of selective drug delivery and minimising toxic effects. A high level of nonspecific internalisation of CS particles was observed in A549 cells with a slight dependence on their concentration (approximately 86-98%). At the same time, CS particles did not show any signs of cytotoxicity toward A549 cells using the XTT assay. The level of internalisation of CS particles with EGF was even higher (93-99%), with no sign of concentration dependence. Thus, EGF-bound chitosan submicroparticles have th	EGF; Chitosan; Submicroparticles; Internalisation; A549; Cell Line