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Calcined Mg-Al Layered Double Hydroxides Have Potential to Reduce Cadmium Toxicity in Zebrafish

Authors: Tichá Lenka | Carneiro Diana | Jelínková Štěpánka | Roušar Tomáš | Martins Roberto | Pavlaki Maria D.

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Calcined Mg-Al Layered Double Hydroxides Have Potential to Reduce Cadmium Toxicity in Zebrafish	Layered double hydroxides (LDHs) are anionic nanoclays consisting of positively charged mixed-metal hydroxide layers intercalated with anions and water molecules. Because of their anionic exchange capacity and adsorption properties, LDHs have attracted attention for their potential in removing pollutants, including heavy metals, from the aquatic environment. Cadmium is one of the metals that can induce adverse biological effects even at low concentrations, particularly in the nervous system. Our study aimed to evaluate the potential of calcined zinc-aluminium LDH-nitrate (c-Zn Al LDH NO3) and calcined magnesium-aluminium LDH-nitrate (c-Mg Al LDH NO3) to prevent cadmium-induced toxicity in zebrafish (Danio rerio) early life stages. We measured mortality, malformations, and hatching rate after exposure of Danio rerio embryos and larvae to cadmium alone and in combination with 100 mg·l-1 c-Zn-Al LDH-NO3 or c-Mg Al LDH-NO3 following the OECD 236 guideline. The molecular changes at DNA level were assessed using a comet assay. Cadmium exposure at tested concentrations after 24-96 h resulted in significant developmental malformations, including pericardial edema, incomplete yolk sac absorption, and tail deformities. Furthermore, DNA damage was observed after 96 h of exposure to the LC50 concentration of cadmium. Notably, co-exposure with c-Mg-Al LDH-NO3 substantially reduced cadmium toxicity, whereas c-Zn-Al LDH-NO3 did not demonstrate a comparable protective effect. These findings suggest that c-Mg-Al LDH-NO3 holds promise as a nanomaterial for reducing cadmium toxicity in aquatic environment. Further testing is needed to unveil why the other nanomaterial was not so effective.	Layered Double Hydroxides; Cadmium; Toxicity; Zebrafish