Publication detail

A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes

Authors: Pešková Iva | Hrubeš Michal | Červenka Libor | Vytřasová Jarmila | Vytřas Karel

Year: 2007

Type of publication: kapitola v odborné knize

Name of source: Sensing in Electroanalysis Vol. 2

Publisher name: Univerzita Pardubice

Place: Pardubice

Page from-to: 201-208

Titles:

Language	Name	Abstract	Keywords
cze	Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody	Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody
eng	A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes	An amperometric method for samonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0,65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella enteritidis in a sample.	Salmonella enteritidis, carbon paste electrode, amperometric detection, alkaline phosphatase.

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