Development of ultrasensitive magneto-immunoassay based on quantum dots/antibody probes for simultaneous electrochemical detection of cancer markers.
Provider: Grantová agentura ČR
Programme: Standardní projekty
Implementation period: 01.01.15 - 31.12.17
Workplace:
Fakulta chemicko-technologická - Katedra biologických a biochemických věd
Investigator: Bílková ZuzanaTeam member: Korecká Lucie | Metelka Radovan
Description:
Project is focused on development of ultrasensitive immunoanalytical method for simultaneous detection of clinically significant markers of ovarian carcinoma (CA125, HE4, CA72-4) in biological material. This multiplex system based on bioaffinity platform with quantum dots as labels and performed in chip in flow mode enables to increase the sensitivity of detection. Analytical approach based on the efficient preconcentration of desired molecules and combined with electrochemical detection by anodic stripping voltammetry is promising tool for diagnosis of disease before the manifestation of clinical symptoms, ideally. Preconcentration unit will consist of magnetic microparticles coated with specific antibodies and fixed by strong magnets in the reaction chamber of microfluidic device. Applied secondary antibodies multilabelled with quantum dots of different materials (Zn, Cd, Pb) specifically captured by immunocomplex become strong source of electrochemical signal after the acidic hydrolysis. For selected cancer biomarkers we assume the limit of detection at the level of ng-pg/ml.
Project is focused on development of ultrasensitive immunoanalytical method for simultaneous detection of clinically significant markers of ovarian carcinoma (CA125, HE4, CA72-4) in biological material. This multiplex system based on bioaffinity platform with quantum dots as labels and performed in chip in flow mode enables to increase the sensitivity of detection. Analytical approach based on the efficient preconcentration of desired molecules and combined with electrochemical detection by anodic stripping voltammetry is promising tool for diagnosis of disease before the manifestation of clinical symptoms, ideally. Preconcentration unit will consist of magnetic microparticles coated with specific antibodies and fixed by strong magnets in the reaction chamber of microfluidic device. Applied secondary antibodies multilabelled with quantum dots of different materials (Zn, Cd, Pb) specifically captured by immunocomplex become strong source of electrochemical signal after the acidic hydrolysis. For selected cancer biomarkers we assume the limit of detection at the level of ng-pg/ml.
