Magnetically active TiO2 nanomaterials suitable for preparation of bioactive proteins and other biopolymers for biotechnological and medical applications
Provider: Technologická agentura České republiky
Programme: Program aplikovaného výzkumu, experimentálního vývoje a inovací GAMA
Implementation period: 01.01.16 - 31.12.17
Workplace:
Fakulta chemicko-technologická - Katedra biologických a biochemických věd
Investigator: Bílková ZuzanaTeam member: Hromádko Luděk | Macák Jan
Description:
The main objective of the project is the development and verification of utility properties of magnetically active TiO2 nanotubes. Project covers the production of the carrier with the declared characteristics (the result of O-type), preparation functional sample (the result of G-type) and the isolation and purification process of selected biomolecules with this carrier (the result of the O-type methodology with proven properties). Methods describing the conditions of the specific interaction of the separated compound with the carrier, conditions for gentle release of the compound from the carrier´s surface to avoid disruption of the biol. activity of the isolated compounds, and finally, the conditions of regeneration and decontamination of carrier for the reusability. Utilization in biotechnology for the preparation of compounds with therapeutic or diagnostic potential is expected. This carrier can be used for the specific isolation of selected biomolecules (rec. proteins with a His-Tag, phosphorylated peptides/proteins as well as nucleic acids ? DNA, RNA) from very complex mixtures with excellent separation characteristics while maintaining very low produce costs for the material. Nanomaterial will be produced in sufficient quantity for demonstration purposes and for a series of qualitative tests of materials and processes. All these findings will be used during the commercialization of the product. Due to high commercialization potential of material both Czech and the PCT patent application is applied and the protection will be also extended by the United States or selected countries of EU. The plan is also to apply another CZ patent application. We will seek for license sale, where we expect interest of companies that provide support to established manufacturers or supply reagents to universities and research institutes. The outputs from the interaction between project team, manufacturers and users of carrier will be used to update the commercialization plan.
The main objective of the project is the development and verification of utility properties of magnetically active TiO2 nanotubes. Project covers the production of the carrier with the declared characteristics (the result of O-type), preparation functional sample (the result of G-type) and the isolation and purification process of selected biomolecules with this carrier (the result of the O-type methodology with proven properties). Methods describing the conditions of the specific interaction of the separated compound with the carrier, conditions for gentle release of the compound from the carrier´s surface to avoid disruption of the biol. activity of the isolated compounds, and finally, the conditions of regeneration and decontamination of carrier for the reusability. Utilization in biotechnology for the preparation of compounds with therapeutic or diagnostic potential is expected. This carrier can be used for the specific isolation of selected biomolecules (rec. proteins with a His-Tag, phosphorylated peptides/proteins as well as nucleic acids ? DNA, RNA) from very complex mixtures with excellent separation characteristics while maintaining very low produce costs for the material. Nanomaterial will be produced in sufficient quantity for demonstration purposes and for a series of qualitative tests of materials and processes. All these findings will be used during the commercialization of the product. Due to high commercialization potential of material both Czech and the PCT patent application is applied and the protection will be also extended by the United States or selected countries of EU. The plan is also to apply another CZ patent application. We will seek for license sale, where we expect interest of companies that provide support to established manufacturers or supply reagents to universities and research institutes. The outputs from the interaction between project team, manufacturers and users of carrier will be used to update the commercialization plan.
