Publication detail

Laccase-functionalized magnetic beads for biotechnology applications

Year: 2008

Type of publication: článek ve sborníku

Name of source: Abstract booklet of 7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers

Publisher name: University of British Columbia

Place: Vancouver

Page from-to: 107

Titles:

Language	Name	Abstract	Keywords
cze	Lakázou modifikované magnetické částice pro biotechnologické aplikace	Enzym Laccase izolovaná z bílé houby fungi Trametes versicolor (T. v.) a Pycnoporus cinnabarinus (P. c.), byla imobilizovaná na magnetické nosiče s výslednou aktivitou 0.22 ? 0.005 I.U./ml resp. 0.63 ? 0.11 I.U./ml sedimentovaného nosiče. Pro stanovení aktivity byly použity substráty Syringaldazin a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). Byla potvrzena výhoda postupu orientované imobilizace.	Lakáza, magnetické částice, biotechnologické aplikace
eng	Laccase-functionalized magnetic beads for biotechnology applications	Enzyme laccase from white rot fungi Trametes versicolor (T. v.) and Pycnoporus cinnabarinus (P. c.), was immobilized among others on magnetic macroporous bead cellulose with activity 0.22 ? 0.005 I.U./ml (random immobilization) and 0.63 ? 0.11 I.U./ml (oriented immobilization) of settled carrier respectively. The oxidation activity of carriers was estimated by syringaldazine and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). The distinct advantage of oriented immobilization has been confirmed.	Laccase, magnetic beads, biotechnology applications

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