Publication detail

Nanofibers with immobilized enzymes - diagnostic material for biotechnology and medicine

Authors: Slováková Marcela | Bílková Zuzana | Juklíčková Martina | Marek Jaromír

Year: 2009

Type of publication: ostatní - přednáška nebo poster

Name of source: Nanofibers for the 3rd Millenium - Nano for Life

Publisher name: Elmarco

Place: Liberec

Page from-to: 114-114

Titles:

Language	Name	Abstract	Keywords
cze	Nanovlákna s imobilizovanými enzymy - diagnostický materiál pro biotechnologii a medicínu	Nanovlákna s imobilizovanými enzymy - diagnostický materiál pro biotechnologii a medicínu	nanovlákna; modifikace enzymy
eng	Nanofibers with immobilized enzymes - diagnostic material for biotechnology and medicine	Polysaccharide film entrapped proteases already produced and tested in mice could be promising for wound therapy. Our lab study covalent immobilizations of different proteases to solid carriers, and the aim of this work was to immobilize trypsin and chymotrypsin on cellulose nanofibers with sufficient enzyme activity, to investigate the effect of immobilization method, temperature, and pH on the preparation, its stability as possible drug delivery system in cutaneous dressings for wound healing. Cellulose as nontoxic, hydrophilic, biocompatible and biodegradable material and possibly nanofibered structure makes it very promising of using as immobilization carrier for drug delivery system. Nanofibers are produced by technology NanospiderTM. Electrospinning is a process capable of producing polymer fibers in nanometer diameter range. After cellulose nanofiber activation (oxidation) and modification by trypsin it showed high proteolytic activity on low-molecular substrate BApNA.	nanofibers; modification; enzymes

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