Publication detail

Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments

Year: 2004

Type of publication: článek v odborném periodiku

Name of source: Journal of Chromatography B

Publisher name: Elsevier Science BV

Place: Amsterdam

Page from-to: 15-24

Titles:

Language	Name	Abstract	Keywords
cze	Využití nově vyvinutých imobilizovaných enzymových reaktorů pro přípravu a studium fragmentů imunoglobulinu G	Využití nově vyvinutých imobilizovaných enzymových reaktorů pro přípravu a studium fragmentů imunoglobulinu G	Imobilizované enzymové reaktory; imunoglobulin G
eng	Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments	The newly developed immobilized enzyme reactors (IMERs) with proteolytic enzymes chymotrypsin, trypsin or papain were used for specific fragmentation of high molecular-mass and heterogeneous glycoproteins immunoglobulin G (IgG) and crystallizable fragment of IgG (Fc). The efficiency of splitting or digestion were controlled by RP-HPLC. The specificity of digestion by trypsin reactor was controlled by MS. IMERs (trypsin immobilized on magnetic microparticles focused in a channel of magnetically active microfluidic device) was used for digestion of the whole IgG molecule. The sufficient conditions for IgG digestion in microfluidic device (flow rate, ratio S:E, pH, temperature) were optimized. It was confirmed that the combination of IMERs with microfluidic device enables efficient digestion of highly heterogeneous glycoproteins such as IgG in extremely short time and minimal reaction volume.	Immobilized enzyme reactors;Immunoglobulin G

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