Login for students

Login for employees

Publication detail

IMERs and IMARs: effective tool for isolation and study of highly heterogeneous glycoproteins
Authors: Korecká Lucie | Ježová Jana | Slováková Marcela | Ouzká Šárka | Bílková Zuzana
Year: 2004
Type of publication: ostatní - přednáška nebo poster
Name of source: 5th International conference on the Scientific and Clinical Applications of Magnetic Carriers
Publisher name: neuveden
Place:
Page from-to:
Titles:
Language Name Abstract Keywords
cze IMER aIMAR: efektivní nástroj pro izolaci a studium vysoce heterogenních glykoproteinů. IMER aIMAR: efektivní nástroj pro izolaci a studium vysoce heterogenních glykoproteinů. IMER; IMAR; magnetické partikule
eng IMERs and IMARs: effective tool for isolation and study of highly heterogeneous glycoproteins The newly developed magnetic micro- and nanoparticles with defined hydrophobicity and porosity (see table) were used for preparation of immobilized magnetic enzyme reactors (IMERs) and immobilized magnetic affinity reactors (IMARs). Magnetic particles with controlled tendency to aggregate and controlled level of nonspecific sorption enable highly efficient and gentle separation during immobilization, high specificity and reproducibility of catalyzed reactions without risk of contamination resulting mixture, elimination of the undesirable dilution and losses of the analyte. Nanometer sized magnetic carriers can be focused in a channel of magnetically active microfluidic analytical device. type of particles material size (ěm) function group developed by concentration immobilized ligand hydrophilic, nonporous alginate coated ferrite microparticles 5-10 -COOH IMCH AS CR 0.5% trypsin hydrophilic, nonporous cellulose magnetite microparticles 6-8 -OH IMCH AS CR 1.2% trypsin, laccase hydrophilic, macroporous bead cellulose 80-100 -OH IMCH AS CR powder papain , protein A hydrophobic, nonporous polyNIPAM 0.29 -COOH Ademtech, France 5% lectins hydrophobic, nonporous polySTYREN 0.86 -NH2 Uptima, France 4% trypsin hydrophobic, nonporous poly(HEMA-co-GMA) (50/50wt) 3.70 -OH IMCH AS CR powder trypsin hydrophobic, nonporous poly(HEMA-co-EDMA-co30%GMA) 3.70 -OH IMCH AS CR powder trypsin hydrophobic, nonporous polyNIPAM, 0.875 -COOH Ademtech, France 2% lectins Table. Specification of used magnetic carriers and its applications The factors as nature of carrier (size, porosity, hydrophilic or hydrophobic character, reactive groups on the surface, behavior of particles in magnetic field, paramagnetism, minimal non-specific adsorption of proteins, peptides) and immobilization procedure (time, temperature, intensity of mixing, environmental pH, rate of enzyme and carrier, necessity of ions, activators, surfactants) were controlled. As important factors ensuring pro IMER; IMAR; magnetic particles

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement