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Magnetic enzyme reactors for isolation and study of heterogeneous glycoproteins.
Authors: Korecká Lucie | Ježová Jana | Bílková Zuzana | Beneš Milan | Horák Daniel | Hradcová Olga | Slováková Marcela | Viovy Jean-Louis
Year: 2005
Type of publication: článek v odborném periodiku
Name of source: Journal of Magnetism and Magnetic Materials
Publisher name: Elsevier Science BV
Place: Amsterdam
Page from-to: 349 ? 357
Titles:
Language Name Abstract Keywords
cze Magnetické enzymové reaktory pro izolaci a studium heterogenních glykoproteinů Nově vyvinuté magnetické mikro-a nanopartikule s definovanou hydrofobicitou a porozitou byly použity pro přípravu magnetických enzymových reaktorů. Magnetické partikule s imobilizovanými enzymy trypsinem, chymotrypsinem, papinem a neuraminidázou byly použity pro studium struktury heterogenních glykoproteinů. Byly optimalizovány parametry, jako je typ nosiče, způsob imobilizace, opeační a skladovací stabilita a experimentální podmínky. Magnetické enzymové reaktory (IMER); Trypsin; Chymotrypsin; Papain; Enzymy; Neuraminidáza; Glykoproteiny; Magnetická separace
eng Magnetic enzyme reactors for isolation and study of heterogeneous glycoproteins. "The newly developed magnetic micro- and nanoparticles with defined hydrophobicity and porosity were used for the preparation of magnetic enzyme reactors. Magnetic particles with immobilized proteolytic enzymes trypsin, chymotrypsin and papain and with enzyme neuraminidase were used to study the structure of heterogeneous glycoproteins. Factors such as the type of carrier, immobilization procedure, operational and storage stability, and experimental conditions were optimized." Magnetic enzyme reactor (IMER); Trypsin; Chymotrypsin; Papain; Enzymes; Neuraminidase; Glycoproteins; Magnetic separation