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New Trends in Qualitative Analysis of Protein
Authors: Bílková Zuzana | Slováková Marcela | Korecká Lucie | Minc Nicolas | Viovy Jean-Louis
Year: 2005
Type of publication: ostatní - přednáška nebo poster
Name of source: 11th International symposium on separation sciences
Publisher name: Univerzita Pardubice
Place:
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Titles:
Language Name Abstract Keywords
cze Nové trendy v kvalitativní analýze proteinů. Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS. trypsin; magnetické mikročástice; Ab (1-40) peptid; epitopová extrakce; imunosorbent; FT-ICR-MS; struktura vakcíny
eng New Trends in Qualitative Analysis of Protein Affinity chromatography utilizes of the specific interactions between two molecules for the purification, determination, identification of a target molecule. In practice, a ligand having the affinity for a target molecule is covalently attached to an insoluble support and functions as bait for capturing the target from complex solutions. Ligands that have been used for affinity separations include small organic compounds that are able to dock into binding sites on proteins, inorganic metals that form coordination complexes with certain amino acids in proteins, hydrophobic molecules that can bind nonpolar pockets in biomolecules, proteins with specific binding regions that are able to interact with other proteins, and antibodies, which can be designed to target any biomolecule through their antigen binding sites. A support or matrix is any material to which a biospecific ligand may be covalently attached. Useful affinity supports are those with a high surface area to volume ration, chemical groups that are easily modified for covalent attachment of ligands, minimal nonspecific binding properties, good flow characteristics and mechanical and chemical stability. The concept of using immobilized affinity ligands has extended beyond just chromatographic applications. Affinity ligand are now coupled to latex beads, nanoparticles, macro-beads, membranes, microplates, array surfaces, dipsticks and a host of other devices, that facilitate the capture of specific biomolecules, proteins. The application of affinity targeting includes purification, scavenging (or removal of contaminants), catalysis (or modification of target molecules) and a broad range of analytical uses, the microspheres have also found use in diagnosis, therapy and drug targeting. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries in high-throughput and standard laboratory applications. Magnetic particles provide universal system with addi trypsin; magnetic microspheres; Ab (1-40) peptide; epitope extraction; immunosorbent; FT-ICR-MS; vaccine lead structure

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