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Chip-based Magnetic Separation Technique Adapted for Qualitative Analysis of Protein
Authors: Bílková Zuzana | Korecká Lucie | Rösnerová Šárka | Slováková Marcela | Minc Nicolas | Viovy Jean-Louis
Year: 2006
Type of publication: ostatní - přednáška nebo poster
Name of source: 17th International Mass Spectrometry Conference
Publisher name: Institute of Microbiology
Place:
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Titles:
Language Name Abstract Keywords
cze Chip-based Magnetic Separation Technique Adapted for Qualitative Analysis of Protein Affinity purification is powerful separation method based on the specific interaction between immobilized ligands and target proteins. Unlike some other chromatography systems, affinity purification can be performed with modified batch methodologies and does not require a packed column. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries in high-throughput and standard laboratory applications. Magnetic particles provide universal system with additional convenience, consistency, stability, ease of handling and exceptional flexibility compared to standard chromatography resins. Implementation of the bioaffinity assays on the microchip format allows faster analysis times due to favorable reaction kinetics on the microscale format. The reduction of sample volume and high-cost reagent consumption are further important priorities. An objective of this presentation is to perform new microsystem using superparamagnetic nanoparticles as a self-organizing matrix. The versatility of new microfluidic device enables integration of different reaction steps in a microchannel. This contribution covers two types of bioaffinity assays: enzymatic assays (specific fragmentation of protein) and isolation of target molecules out of complex matrix (tryptic peptides purification). chip, magnetic particles, protein
eng Chip-based Magnetic Separation Technique Adapted for Qualitative Analysis of Protein Affinity purification is powerful separation method based on the specific interaction between immobilized ligands and target proteins. Unlike some other chromatography systems, affinity purification can be performed with modified batch methodologies and does not require a packed column. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries in high-throughput and standard laboratory applications. Magnetic particles provide universal system with additional convenience, consistency, stability, ease of handling and exceptional flexibility compared to standard chromatography resins. Implementation of the bioaffinity assays on the microchip format allows faster analysis times due to favorable reaction kinetics on the microscale format. The reduction of sample volume and high-cost reagent consumption are further important priorities. An objective of this presentation is to perform new microsystem using superparamagnetic nanoparticles as a self-organizing matrix. The versatility of new microfluidic device enables integration of different reaction steps in a microchannel. This contribution covers two types of bioaffinity assays: enzymatic assays (specific fragmentation of protein) and isolation of target molecules out of complex matrix (tryptic peptides purification). chip, magnetic particles, protein

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