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Use of self-assembled magnetic beads for on-chip protein analysis
Authors: Bílková Zuzana | Slováková Marcela | Korecká Lucie | Minc Nicolas | Viovy Jean-Louis
Year: 2006
Type of publication: ostatní - přednáška nebo poster
Name of source: 20th International Symposium on MicroScale Biopseparation
Publisher name: HPCE 2006 Foundation
Place:
Page from-to:
Titles:
Language Name Abstract Keywords
cze Use of self-assembled magnetic beads for on-chip protein analysis There is a continuous push to miniaturize and integrate as many laboratory instrumentation pieces as possible in the quest for a ?lab-on-chip?. Three of the most important advantages of using microfluidic system of reduced dimension for analytical applications are known to be: 1) using minute quantities of sample and reagents (micro-,nanoliters), 2) relatively fast reaction times when molecular diffusin lengths are of the order of the microchannel dimension, 3) a large surface-to-volume ratio offering an intrinsic compatibility between the use of a microfluidic system and surface-based assays 1. Parallel to the boom of microfluidic systems, nanomaterials and nanoparticles with paramagnetic properties have become a hot topic in recent research. Functional nano- and microparticles offer a large specific surface for ligand binding. The concept of using immobilized ligands has extended beyond just chromatographic applications. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries in high-throughput and standard laboratory applications. Magnetic particles provide universal system with additional convenience, consistency, stability, ease of handling and exceptional flexibility compared to standard chromatography resins. Verpoorte (2003) 2 and Gijs (2004) 1 recently reviewed new technology based on the integration of magnetic functionalized carriers within microfluidic systems. Microanalytical system with packed plug of bio-active beads profit from a larger surface-to-volume ratio, faster analysis time due to favorable reaction kinetics on the microscale format. Furthermore, integration of several assay functions on a single chip leads to assay automation and elimination of operator involvement as a variable. An objective of this presentation is to perform new microfluidic analytical system using superparamagnetic nanoparticles as a self-organizing matrix. T magnetic beads, on-chip protein analysis
eng Use of self-assembled magnetic beads for on-chip protein analysis There is a continuous push to miniaturize and integrate as many laboratory instrumentation pieces as possible in the quest for a ?lab-on-chip?. Three of the most important advantages of using microfluidic system of reduced dimension for analytical applications are known to be: 1) using minute quantities of sample and reagents (micro-,nanoliters), 2) relatively fast reaction times when molecular diffusin lengths are of the order of the microchannel dimension, 3) a large surface-to-volume ratio offering an intrinsic compatibility between the use of a microfluidic system and surface-based assays 1. Parallel to the boom of microfluidic systems, nanomaterials and nanoparticles with paramagnetic properties have become a hot topic in recent research. Functional nano- and microparticles offer a large specific surface for ligand binding. The concept of using immobilized ligands has extended beyond just chromatographic applications. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries in high-throughput and standard laboratory applications. Magnetic particles provide universal system with additional convenience, consistency, stability, ease of handling and exceptional flexibility compared to standard chromatography resins. Verpoorte (2003) 2 and Gijs (2004) 1 recently reviewed new technology based on the integration of magnetic functionalized carriers within microfluidic systems. Microanalytical system with packed plug of bio-active beads profit from a larger surface-to-volume ratio, faster analysis time due to favorable reaction kinetics on the microscale format. Furthermore, integration of several assay functions on a single chip leads to assay automation and elimination of operator involvement as a variable. An objective of this presentation is to perform new microfluidic analytical system using superparamagnetic nanoparticles as a self-organizing matrix. T magnetic beads, on-chip protein analysis

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