Publication detail

Different proteomic approaches to analysis of Francisella tularensis bacterial glycoproteins

Year: 2007

Type of publication: ostatní - přednáška nebo poster

Name of source: Journal of American Society for Mass Spectrometry

Publisher name: Elsevier Science Inc.

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
cze	Různé proteomické postupy pro analýzu bakteriálních glykoproteinů Francisella tularensis	Různé proteomické postupy pro analýzu bakteriálních glykoproteinů Francisella tularensis	Francisella tularensis; membránové glykoproteiny; MS
eng	Different proteomic approaches to analysis of Francisella tularensis bacterial glycoproteins	Two subspecies of Francisella tularensis were analysed: the highly virulent subspecies tularensis strain SCHU S4 and a less virulent subspecies holarctica strain LVS. We find differences in detection of membrane-localized glycoproteins between the strains. Ten spots were detected on the LVS gel and five proteins with/without their protein species (PS) were identified by mass spectrometry (conserved hypothetical lipoproteins FTT1103 - two PS, OMPA family protein FTT0831 ? three PS, intracellular growth locus, subunit B FTT1358, outer membrane associated protein FopA FTT 0583 ? three PS, hypothetical membrane protein FTT1676). By contrast, seven spots were detected on the SCHU S4 gel and three proteins with/without their protein species were identified (FTT1103, FTT0831 ? three PS, FTT 0583 ? three PS). We used bioinformatics tools (the NetOglyc server) for evaluation of results.	Francisella tularensis; membrane glycoproteins; MS

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