University of PardubiceFaculty of Chemical Technology

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Fabrication and enzymatic biofunctionalization of nanofibers as a new material for wound dressings

Authors: Slováková Marcela | Kučerová Jana | Juklíčková Martina | Stránská Denisa | Korecká Lucie | Holubová Lucie | Bílková Zuzana | Vlček Milan

Year: 2010

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
cze	Výroba a enzymatická biofunkcionalizace nanovláken jakožto nový materiál pro krytí ran	V posledních letech se nanomateriály staly velmi populárním materiálem nejen ve světě techniky ale i na poli biomedicíny. Naším ukolem bylo vyrobit celulózová a chitosanová nanovlákna a biofunkcionalizovat je lysozymem a trypsinem. Nanovlákna byla vyrobena technologií Nanospider TM, unikátní technologií založenou na procesu electrospinningu, který umožnuje vyrábět nanovlákenné textilie v průmyslovém měřítku. Výrobní parametry byly optimalizovány tak, aby bylo dosaženo požadovné váhy a průměru nanovláken.	nanovlákna; enzymy
eng	Fabrication and enzymatic biofunctionalization of nanofibers as a new material for wound dressings	In past years nanomaterials have become one of the most popular scientific area not only in technical field but also in biomedicine. Goal of our work was to fabricate cellulose and chitosan nanofibers and to biofunctionalize them by lysozyme and trypsin. Nanofibers were prepared by technology NanospiderTM, a unique technology based on electrospinning process that enables production of nanofibrous textiles on an industrial scale. The fabrication parameters were optimized for obtaining desired weight area and diameter of nanofibers. For both enzymes the bond formation and enzyme stability was firstly pretested on model system with magnetic cellulose microparticles (MPs). After that the enzymes (lysozyme, trypsin) were covalently immobilized on the surface of nanofibers and the enzymatic activity was measured. Our first results indicate that biofunctionalized nanofibers could be a new promising approach for wound treatment.	nanofibers; enzymes; wound dressings