Publication detail

Microchip Electrophoresis Profiling of Abeta Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease

Year: 2010

Type of publication: článek v odborném periodiku

Name of source: Analytical Chemistry

Publisher name: American Chemical Society

Place: Washington

Page from-to: 7611-7617

Titles:

Language	Name	Abstract	Keywords
cze	Profilování Abeta peptidů v mozkomišním moku pacientů s Alzheimerovou chorobou pomocí elektroforézy v čipu	Jednou z hlavních neuropatologických projevů Alzheimerovy choroby (AD) je agregace Abeta 1-42 do amyloidových plaků. Byla popsána gelovou elektroforézu v čipu (PDMS), který umožňuje rychlé profilování Abeta peptidů v mozkomišním moku pacientů s AD.	Mikročip; Elektroforéza; Mozkomišní mok; Alzheimerova choroba; Imunosorbent; Magnetické částice
eng	Microchip Electrophoresis Profiling of Abeta Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease	The preferential aggregation of A1-42 in amyloid plaques is one of the major neuropathological events in Alzheimer’s disease. Here, we describe a microchip gel electrophoresis method in polydimethylsiloxane (PDMS) chip that enables rapid profiling of major A peptides in cerebrospinal fluid. To control the electroosmotic flow (EOF) in the PDMS channel and also to reduce the adsorption of the peptides to the surface of the channel, a new double coating using poly(dimethylacrylamide-co-allyl glycidyl ether) (PDMA-AGE) and methylcellulose-Tween-20 was developed.	Microchip; Electrophoresis; Cerebrospinal Fluid; Alzheimer’s Disease; Immunosorbent, Magnetic Particles

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