University of PardubiceFaculty of Chemical Technology

University

Employees
|
Students
Česky

Publication detail

In-gel detection of phosphoproteins by selective staining: possibilities and comparison

Authors: Jankovičová Barbora | Kroupa Tomáš | Špidlová Petra | Korecká Lucie | Hernychová Lenka | Bílková Zuzana

Year: 2010

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	In-gel detection of phosphoproteins by selective staining: possibilities and comparison	Protein phosphorylation represents one of the most studied post-translational modifications, which in particular plays a significant role in a wide range of cellular processes. In this work we focused on selective detection of phosphoproteins directly in 1-D or 2-D electrophoretic gels using different staining techniques. We compared commercially available fluorescent detection by Pro-Q Diamond (Invitrogen) and Phos-tag Phosphoprotein Gel Stain (PerkinElmer) and non-fluorescent GelCode Gel Stain (Pierce). Mentioned dyes were utilized for selective detection of phosphoproteins in model protein mixtures, cell lysates of Francisella tularensis or in dephosporylation studies. Parameters such selectivity, sensitivity, required instrumentation, time-consumption or compatibility with MALDI-TOF-MS were evaluated.	In-gel detection; phosphoproteins; selective staining