University of PardubiceFaculty of Chemical Technology

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Peptide mapping by CZE of tau protein, a biomarker of Alzheimer´s disease

Authors: Riviere Celine | Bohoyo Diego | Taverna Myriam | Klafki Hans | Wiltfang Jens | Jankovičová Barbora | Slováková Marcela | Bílková Zuzana | Poirier Florence | Le Potier Isabelle

Year: 2010

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Peptide mapping by CZE of tau protein, a biomarker of Alzheimer´s disease	Alzheimer´s disease (AD) is a neurodegenerative disorder that causes progressive loss of mental functions. Established biomarkers for AD are elevated levels of Tau protein and reduced amyloid-beta (1-42) peptides in CSF. Hyperphosphorylated tau isoforms are the major component of the neurofibrillary lesions found in AD and other tauopathies. Our project intends to develop an analytical methotology based on CE to quantify in biological fluids, the ratio between phosphorylated and normal Tau protein. Our approach has been focus on fragments of tau, obtained after an optimized procedure of chemical cleavage. One of the six theoretically generated peptides is expected to be common to all the Tau isoforms. We have used the lab on chip bioanalyser and developed CZE conditions to monitor not only the cleavage efficiency on tau, but also to establish a high resolution peptide mapping of this protein. Finally differences in the CE profile for normal and phosphorylated Tau were highlighted.	CZE; tau protein; Alzheimer´s disease