Publication detail

New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.

Year: 2012

Type of publication: článek v odborném periodiku

Name of source: Macromolecular Bioscience

Publisher name: Wiley-Blackwell

Place: Oxford

Page from-to: 647-655

Titles:

Language	Name	Abstract	Keywords
cze	Nové monodisperzní magnetické polymerní mikročástice biofunkcionalizované pro enzymovou katalýzu a bioafinitní separace	Magnetické makroporézní PGMA a PHEMA mikročástice obsahující karboxylové funkční skupiny byly syntetizovány pomocí vícekrokové polymerizační metody následované precipitací oxidů kovů uvnitř póru připravených mikročástic. Využití částic v enzymové katalýze a bioafinitních separacích bylo potvrzeno imobilizací trypsinu a huIgG na povrch těchto nosičů.	enzymová katalýza; glycidyl metakrylát; 2-hydroxyethyl metakrylát
eng	New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.	Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.	enzyme catalysis; glycidyl methacrylate; 2-hydroxyethyl methacrylate

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