University of PardubiceFaculty of Chemical Technology

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Surface Modification of Magnetic Particles with Hyaluronic Acid for Minimalization of Non-Specific Sorption

Authors: Holubová Lucie | Palarčík Jiří | Knotek Petr | Bělina Petr | Korecká Lucie | Bílková Zuzana

Year: 2012

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Surface Modification of Magnetic Particles with Hyaluronic Acid for Minimalization of Non-Specific Sorption	The development of systems based on the integration of biofunctionalized carriers within microfluidic device is nowadays in considerable interest. Microanalytical systems with packed plug of bioactive carriers profit from a minimal quantities of sample and reagents, a larger surface-to-volume ratio, faster analysis time due to improvement of reaction kinetics and integration of different reaction steps. Carriers with superparamagnetic properties provide universal system with additional convenience, consistency, stability, easy of handling and exceptional flexibility. The problem of their application lies in non-specific sorption to the different types of surfaces. Polysaccharide coating could serve as potential low-fouling surface modifier which reduces sorption to channel. Compare to other types of polysaccharides, hyaluronic acid (HA) has received much attention due to its unique properties. HA is present in almost all biological fluids and tissues and its biological characteristics are exploited to non-specific sorption minimalization, increase stability of particles, improve physical and chemical properties, achieve biocompatibility of systems and obtain high binding capacity for immobilization of other bioactive ligands. Surface modification technique was developed using commercial magnetic microparticles. The method of covalent binding was applied to ensure stability and minimal HA-layer disruption, in contrast to common adsorption techniques. The binding conditions were optimized to achieve maximal amount of bound HA and high layer stability. Efficiency of surface modification was verified using non-spectral methods including zeta potential and particle size measurement, spectroscopic and microscopic methods. The presence of HA-layer and efficiency of surface modification technique were confirmed. The HA-coated microparticles will be consequently biofunctionalized with various ligands and applied into the microfluidic device for purpose of interest.	magnetic particles; microfluidic device; hyaluronic acid; surface modification; non-specific sorption