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Albumin-coated monodisperse magnetic poly(glycidyl methacrylate) microspheres with immobilized antibodies: Application to the capture of epithelial cancer cells
Authors: Horák Daniel | Svobodová Zuzana | Autebert Julien | Coudert Benoit | Plichta Zdeněk | Královec Karel | Bílková Zuzana | Viovy Jean-Louis
Year: 2013
Type of publication: článek v odborném periodiku
Name of source: Journal of Biomedical Materials Research - Part A
Publisher name: Wiley-Blackwell
Page from-to: 23-32
Titles:
Language Name Abstract Keywords
cze Monodisperzní magnetické poly(glycidyl methacrylátové) částice potažené albuminem s imobilizovanými protilátkami: Aplikace na izolaci epiteliálních nádorových buněk Článek popisuje podrobně přípravu magnetických částic, jejich potažení albuminem pro snížení nespecifické sorpce a také zavádění funkčních skupin pro vazbu protilátek na jejich povrch, tedy přípravu magnetického imunosorbentu. Zároveň je zde popsána jejich aplikace v mikrofluidním zařízení Magnetismus; mikročástice; buňky
eng Albumin-coated monodisperse magnetic poly(glycidyl methacrylate) microspheres with immobilized antibodies: Application to the capture of epithelial cancer cells Monodisperse (4 ?m) macroporous crosslinked poly(glycidyl methacrylate) microspheres for use in microfluidic immunomagnetic cell sorting, with a specific application to the capture of circulating tumor cells (CTCs), were prepared by multistep swelling polymerization in the presence of cyclohexyl acetate porogen and hydrolyzed and ammonolyzed. Iron oxide was then precipitated in the microspheres to render them magnetic. Repeated precipitation made possible to raise the iron oxide content to more than 30 wt %. To minimize non-specific adsorption of the microspheres in a microchannel, and of cells on the microspheres, they were coated with albumin crosslinked with glutaraldehyde. Antibodies of epithelial cell adhesion molecule (anti-EpCAM) were then immobilized on the albumin-coated magnetic microspheres using the carbodiimide method. Capture of MCF7 cells as a model of CTCs by the microspheres with immobilized anti-EpCAM IgG was performed in a batch experiment. Finally, MCF7 cells were captured by the anti-EpCAM-immobilized albumin-coated magnetic microspheres in an Ephesia chip. A very good rejection of lymphocytes was achieved. Thus, albumin-coated monodisperse magnetic poly(glycidyl methacrylate) microspheres with immobilized anti-EpCAM seem to be promising for capture of circulating tumor cells in a microfluidic device. Magnetism; Microsphere; Cells

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