University of PardubiceFaculty of Chemical Technology

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PEGylation as a tool for enhancing the quality of magnetic immunosorbents used in microfluidic devices

Authors: Kučerová Jana | Svobodová Zuzana | Horák Daniel | Autebert Julien | Palarčík Jiří | Bílková Zuzana | Viovy Jean-Luis

Year: 2012

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	PEGylation as a tool for enhancing the quality of magnetic immunosorbents used in microfluidic devices	Magnetic poly (glycidyl methacrylate) monodisperse microspheres with terminal functional carboxyl group were prepared by multistep swelling and polymerization method. The prepared microbeads were porous, 3.9 μm in size and carboxyl content was 0.2 mmol.g-1. Despite their highly hydrophilic character they strongly adhered to the PDMS microchannel. The surface of the microspheres was therefore modified with heterobifunctional poly (ethylene glycol) (PEG). The presence of PEG was confirmed by biotin-streptavidin-based sandwich assay and by zeta potential measurements. PEG served simultaneously as a spacer for subsequent antibodies conjugation, which could increase their steric accessibility and affinity of immobilized antibody to the surface antigen.	PEGylation; magnetic microspheres; microfluidics; antibodies