Login for students

Login for employees

Publication detail

Determination of Inhibition Efficiency of Carbamates Inhibitors of Acetylcholinesterase
Authors: Vorčáková Katarína | Štěpánková Šárka | Zorič Petra | Štramová Xenie
Year: 2013
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Determination of Inhibition Efficiency of Carbamates Inhibitors of Acetylcholinesterase Alzheimer´s disease is an age dependent neurodegenerative disorder that destroys brain cells, causes problems with memory, thinking and behaviour. There are many hypotheses of the etiology of this disease. The cholinergic hypothesis relates with increased activity of acetylcholinesterase (ACHE) and the neurodegeneration with the loss of cholinergic neurotransmission. ACHE is an enzyme primarily responsible for the rapid elimination of acetylcholine (ACH), within one millisecond after its release at cholinergic synapses. Nowadays, for treatment of AD inhibitors of ACHE are used. Ellman´s method (ELM) is based on measuring of yellow product which is formed in reaction between the thiol reagent 5,5?-dithiobis-2-nitrobenzoic acid and thiocholine (TCH), a product of substrate (acetylthiocholine, ATCH) hydrolysis by ACHE. Final concentration of ATCH in reaction mixture was 0.4 ?M. The electrochemical method is based on measuring of anodic oxidation current of TCH using square-wave voltammetry. Final concentration of ATCH in reaction mixture was 1 mM. The third one, pH-stat method is based on determination of actual concentration of acetic acid (HA) by continuous titration of HA with analytical solution of 0.1 M potassium hydroxide. Final concentration of ACH in reaction mixture was 1 mM. All measurements were carrying out with the final activity of ACHE in reaction mixture 0.2 U. For each measurement, the concentration of inhibitor was chosen experimentally so as to be visible inhibitory effect (slow hydrolysis). Inhibiting efficiency characterized by IC50 (concentration of inhibitor that causes a reduction of rate of inhibited reaction to one half compared to uninhibited reaction) was calculated. To the according data, the most effective inhibitor is 4-chlorobenzyl phenylcarbamate. Carbamates; Inhibitors; Acetylcholinesterase

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0Creative CommonsAttributionNonCommercialNoDerivatives