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Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation of Salmonella
Authors: Kučerová Jana | Holubová Lucie | Bílková Zuzana
Year: 2013
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation of Salmonella The selection of antibodies with the desired specificity, immunoreactivity, purity and integrity is a key step in developing an effective immunosorbent for immunomagnetic separation. Four commercial anti-Salmonella antibodies were evaluated from the point of view of their immunoreactivity with the cells of Salmonella Typhimurium and of their purity. For these purposes, traditional SDS-PAGE analysis with subsequent silver staining and a whole-cell dot blot technique were applied. Salmonella; immunomagnetic separation; antibodies; dot blot;