Login for students

Login for employees

Publication detail

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays
Authors: Srbová Jana | Svobodová Zuzana | Knotek Petr | Palarčík Jiří | Vlček Milan | Kincl Miloslav | Horak Daniel | Autebert Julien | Viovy Jean-Louis | Bílková Zuzana
Year: 2014
Type of publication: článek v odborném periodiku
Name of source: Materials Science & Engineering C: Materials for Biological Applications
Publisher name: Elsevier Science BV
Place: Amsterdam
Page from-to: 308-315
Titles:
Language Name Abstract Keywords
cze PEGylace magnetických poly(glycidyl methakrylátových) mikročástic pro biomikrofluidiku Magnetické poly(glycidyl methakrylátové) mikročástice s karboxylovými skupinami byly funkcionalizovány vysoce hydrofilním polymerem poly(ethylen glykolem) (PEG). PEG byl použit pro snížení nespecifických interakcí s proteiny a buňkami, zároveň snižoval adhezi částic na stěny mikrofluidních zařízení z poly(dimethylsiloxanu) (PDMS) a z cyklického kopolymeru olefinu (COC). Pro průkaz PEGu na povrchu částic bylo využito měření zeta potenciálu, infračervená spektroskopie, skenovací elektronová mikroskopie, ELISA s anti-PEG protilátkami a bioafinitní interakce využívající reakce biotinu a streptavidinu. Po pokrytí částic PEGem byly částice inkubovány v přítomnosti inertního proteinu bovinního sérového albuminu a buněk. Poté byla hodnocena míra nespecifické adsorpce proteinu a buněk na povrch částic. PEGylace; Magnetické mikročástice; mikrofluidika; nespecifická adsorpce
eng PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly( ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC). Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, and bioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres. Both neat and PEGylated microspheres were then incubated with the inert protein bovine serum albumin or cells to evaluate the rate of nonspecific adsorption (NSA). PEG with Mr of 30,000 Da was responsible for 45% reduction in NSA of proteins and 74% for cells compared to neat particles. The microspheres' behavior in PDMS and COC microchannels was then evaluated. Aggregation and adhesion of PEGylated microspheres significantly decreased compared to neat particles. Finally, the model enzyme horseradish peroxidase was immobilized on the microspheres through the heterobifunctional PEG chain. The possibility for subsequent covalent coupling of the ligand of interest was confirmed. Such PEGylated microparticles can be efficiently used in PDMS microchips as a carrier for bioaffinity separation or of enzyme for catalysis. PEGylation; Magnetic microspheres; Microfluidics; Nonspecific adsorption

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0Creative CommonsAttributionNonCommercialNoDerivatives