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Interaction of tumor cells with antibodies against human carbonic anhydrase I

Authors: Kročová Eliška | Jankovičová Barbora | Dvořáková Veronika | Brůčková Lenka | Lakota Ján | Bílková Zuzana

Year: 2014

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Interaction of tumor cells with antibodies against human carbonic anhydrase I	There is a group of patients with different types of malignancies who developed an aplastic anemia like syndrome after a high-dose-therapy and autologous stem cell transplantation, but even though they appeared to have spontaneous tumor regression without relapse. It was found that these patients have high titers of serum autoantibodies against carbonic anhydrase, isoform I (anti-CAI) and the presence of the anti-CAI is therefore associated with malignancy regression [1]. This study is focused on monitoring of the interactions between anti-CAI antibodies and tumor cells. Especially we study the influence of these antibodies on viability of tumor cells by xCELLigence and then we try to reveal the mechanism of action of this process by identification of proteins, which react with anti-CAI and could be involved in the process. For this we used the interactions between anti-CAI antibodies and tumor cells lysates analyzed by two-dimensional gel electrophoresis followed by western blot analysis. The experiments demonstrated that the antibodies interact not only with their specific antigen (CAI) and that they affect cell grow of tested tumor cell line. It could explain the effect of repression on tumor cells and clarify the phenomenon of an aplastic anemia and spontaneous tumor regression in the patients.	Carbonic anhydrase I, autoimmunity, autoantibody