University of PardubiceFaculty of Chemical Technology

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„in-house“ labeling protocol for routine preparation of quantum dot-based conjugates Suited for electrochemical detection

Authors: Dvořáková Veronika | Čadková Michaela | Datínská Vladimíra | Chalupniak Andrzej | Merkoci Arben | Klepárník Karel | Foret František | Bílková Zuzana | Korecká Lucie

Year: 2015

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	„in-house“ labeling protocol for routine preparation of quantum dot-based conjugates Suited for electrochemical detection	Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.	quantum dots; conjugation protocol; IgG molecules