Publication detail

ELECTROCHEMICAL DETECTION OF OVARIAN CANCER BIOMARKER HE4 BASED ON µQLISA PRINCIPLE

Authors: Čadková Michaela | Dvořáková Veronika | Korecká Lucie | Bílková Zuzana

Year: 2015

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	ELECTROCHEMICAL DETECTION OF OVARIAN CANCER BIOMARKER HE4 BASED ON µQLISA PRINCIPLE	Electrochemical immunosensors in connection with quantum dots (QDs) represent smart devices which provide high sensitivity and specificity for detection of target analytes. However, the unavailability of commercial specific antibodies labelled by QDs result in the need of the introduction of various labeling methods. The main goal of the work was to implement new approach for antibody multilabeling alowing detection of extremely low levels of biomarkers, typical especially for early stage cancerous diseases. This technique essentially uses carbodiimide chemistry combined with magnetic bead-based separation. Proposed protocol brings several benefits such as protection of antibody active site, low costs, high purity and ready-to-use character comparing to commercially available kits.	electrochemical immunosensor; HE4; ovarian cancer

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